P-104 Impact of Semen Microbiota on the Embryo Quality

Abstract Study question Is there a relationship between the microbiota composition of semen samples used for assisted reproductive technologies (ART) and quality embryos? Summary answer The obligate anaerobes prevalence in was associated with reduced embryo patients astenozoospermia, but no difference found normozoospermic samples. What is known already success ART depends on number factors, including quality. viability IVF could be affected by various environmental contamination microbiota. Semen not sterile, even processed it may contain some bacteria, which colonize culture dishes oocytes embryos. It shown that presence bacteria might alter sperm poor developing design, size, duration 126 infertile couples attending “Garmonia” Medical Center (Yekaterinburg, Russia) 2020-2021, were included study. Depending spermiogram results, they divided into two groups Group 1 (n = 51) — asthenozoospermia, 2 75) normospermia. Participants/materials, setting, methods analyzed using RT-PCR kit Androflor (DNA-Technology, Russia). Cluster analysis performed 78 total bacterial load (TBL) at least 103 GE/ml (asthenozoospermia 31, normozoospermia 47). conducted k-means ++ algorithm, scikit-learn. blastocyst development rate (BDR), as proportion 2PN zygotes, determined day 5 after injection. BDR ≥ 40% considered satisfactory, < - unsatisfactory. Main results role chance When analyzing microbiota, 8 stable clusters distinguished. lactobacilli-dominated, 3 Gram-positive facultative dominated Corynebacterium spp. Streptococcus respectively; 4 –Enterobacteriaceae/Enterococcus spp-dominated,; 5, 6 Bacteroides spp./Porphyromonas spp./Prevotella or Peptostreptococcus spp./Parvimonas spp., respectively. 7 formed mixed (MOA); Mycoplasmae. There detection individual normospermia asthenozoospermia. their each group subgroups: Subgroup 1A 25) 40%, 1B 26) 40%; 2A 38) 2B 37) 40%. different Subgroups (normospermia samples) almost similar. In asthenozoospermia lower (Subgroup 1B) higher (53.8%) comparing to those (16.0%), p 0.001. detected more often (20.0%) than (7.8%), > 0.05. TBL present 52% 69.2% Limitations, reasons caution GE/ml, since incompatible data received negative control Wider implications findings Further research determine significance described other pathologies. Establishing characteristics allow new algorithms treating disorders, depending Trial registration applicable